Music is a tradition at Greenville not limited to music majors. For over 85 years the Greenville College Choir has taken an annual spring tour in addition to weekend trips. Membership is by audition, and voice lessons are encouraged. This elite vocal group has a reputation for fine choral performances in music ranging from Bach to contemporary composers. In 2010, the College began a Marching and Pep Band. Attenders of sporting events and homecoming have enjoyed showing their Panther Spirit alongside these two groups. The College Band has also established its place as a performing group. Within the concert band are several ensembles. Other opportunities for musical involvement are available with Chamber Singers, Chorale, and Chamber Orchestra.

The College has a preeminent contemporary Christian music program, and students completing this curriculum receives a Bachelor of Science degree with a major in Commercial Music. The Department sponsors a number of worship arts ensembles. Each semester several lab bands are chosen by audition.